A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space

Microfluidic Tools to Investigate Protein Crystallization

Microfluidic Tools to Investigate Protein Crystallization A dissertation presented to the Faculty of the Graduate School of Arts and Sciences of Brandeis University, Waltham, Massachusetts by Michael Heymann There is no guarantee that a given protein has a crystalline phase, but even existence of an equilibrium crystalline phase is not sufficient for a crystal to form because the transformation...

Crystallization of Sub-Micrometer Sized ZSM-5 Zeolites in SDA-Free Systems

A complete microfluidic screening platform for rational protein crystallization.

By virtue of their unique mass transport properties, dense integration, and low reagent consumption, microfluidic devices have emerged as viable technologies for protein crystallization. Current microfluidic crystallization technologies have focused on two separate strategies: one using the large-scale integration of active microvalves,1 and one using two-phase flows for the mixing and encapsul...

Protein crystallization

This essay is about the crystallization of proteins. Since the first published observation of crystallizing proteins about 160 years ago, protein crystal growth has developed into an extensive research field with many applications, for instance in the pharmaceutical industry. In this report, an overview of the unique physical-chemical characteristics of protein molecules, their properties in so...

Protein crystallization in vivo

Protein crystallization in vivo provides some fascinating examples of biological selfassembly. Here, we provide a selective survey to show the diversity of functions for which protein crystals are used, and the physical properties of the crystals that are exploited. Where known, we emphasize how the nature of the protein-protein interactions leads to control of the crystallization behaviour.